Journal: Cell stem cell

Article Title: Vimentin coordinates protein turnover at the aggresome during neural stem cell quiescence exit

doi: 10.1016/j.stem.2020.01.018

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Harm Kampinqa N/A Tdp43-gfp overexpression Addgene Cat#28197 mNeon overexpression Gift from Dr. Erik Dent, mNeon cDNA from Allele Biotechnology & Pharmaceuticals, Inc N/A VHL-BFP overexpression This paper, VHL insert from Addgene #21053 N/A GFP-Ubiquitin Addgene Cat#11928 mCherry-GFP-LC3 Gift from Dr. Ashley Webb N/A psPAX2 Addgene Cat#12260 pCMV-VSV-G Addgene Cat#8454 Software and Algorithms Microsoft Excel Microsoft N/A Prism GraphPad N/A Adobe Illustrator Adobe.com N/A Imaris Oxford Instruments N/A Other N/A N/A N/A Open in a separate window KEY RESOURCES TABLE EXPERIMENTAL MODEL AND SUBJECT DETAILS Mice In this study, male and female mice between the ages of 6 weeks and 9 months were used.

Techniques: Immunofluorescence, Western Blot, Proximity Ligation Assay, Recombinant, SYBR Green Assay, RNA Sequencing Assay, Clone Assay, Over Expression, Software

Journal: Cell stem cell

Article Title: Vimentin coordinates protein turnover at the aggresome during neural stem cell quiescence exit

doi: 10.1016/j.stem.2020.01.018

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: To construct CAG-BFP-VHL, a four piece Gibson assembly was performed ligating eBFP from CMV-eBFP-2A-H2B-eGFP, VHL-WPRE from pESC-LEU-GFP-VHL (Addgene #21053) and a linker between VHL and BFP into a CAG-GFP-PRE vector ( Kaganovich et al., 2008 ).

Techniques: Immunofluorescence, Western Blot, Proximity Ligation Assay, Recombinant, SYBR Green Assay, RNA Sequencing Assay, Clone Assay, Over Expression, Software

Journal: Cell stem cell

Article Title: Vimentin coordinates protein turnover at the aggresome during neural stem cell quiescence exit

doi: 10.1016/j.stem.2020.01.018

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Harm Kampinqa N/A Tdp43-gfp overexpression Addgene Cat#28197 mNeon overexpression Gift from Dr. Erik Dent, mNeon cDNA from Allele Biotechnology & Pharmaceuticals, Inc N/A VHL-BFP overexpression This paper, VHL insert from Addgene #21053 N/A GFP-Ubiquitin Addgene Cat#11928 mCherry-GFP-LC3 Gift from Dr. Ashley Webb N/A psPAX2 Addgene Cat#12260 pCMV-VSV-G Addgene Cat#8454 Software and Algorithms Microsoft Excel Microsoft N/A Prism GraphPad N/A Adobe Illustrator Adobe.com N/A Imaris Oxford Instruments N/A Other N/A N/A N/A Open in a separate window KEY RESOURCES TABLE

Techniques: Immunofluorescence, Western Blot, Proximity Ligation Assay, Recombinant, SYBR Green Assay, RNA Sequencing Assay, Clone Assay, Over Expression, Software